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Inhibition of the TEF/TEAD transcription factor
activity by nuclear calcium and distinct kinase
pathways.
Thompson M, Andrade VA, Andrade SJ, Pusl
T, Ortega JM, Goes AM, Leite MF.
Department of Physiology and
Biophysics, Federal University of Minas Gerais, Belo Horizonte, MG
31270-901, Brazil.
Transcription enhancer factor (TEF/TEAD) is a
family of four transcription factors that share a common TEA-DNA binding
domain and are involved in similar cellular functions, such as cell
differentiation and proliferation. All adult tissues express at least
one of the four TEAD genes, so this family of transcription factors may
be of widespread importance, yet little is known about their regulation.
Here we examine the factors that regulate TEAD activity in CHO cells.
RT-PCR indicated the presence of TEAD-1, TEAD-3, and both isoforms of
TEAD-4, but not TEAD-2. Quantitative measurements showed that TEAD-4 is
most abundant, followed by TEAD-3, then TEAD-1. We examined the relative
effects of nuclear and cytosolic Ca(2+) on TEAD activity, since TEAD
proteins are localized to the nucleus and since free Ca(2+) within the
nucleus selectively regulates transcription in some systems. Chelation
of nuclear but not cytosolic Ca(2+) increased TEAD activity two times
above control. Inhibition of mitogen-activated protein kinase (MAPK)
also increased TEAD activity, while cAMP decreased TEAD activity, and
protein kinase C had no effect. Together, these results show that
nuclear Ca(2+), MAPK, and cAMP each negatively regulate the activity of
the TEAD transcription factor.
PMID: 12565854 [PubMed - indexed
for MEDLINE]
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