ProtParam tool

User-provided sequence:

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAVRVAINGF GRIAVTILRA IVESGRTDIQ VVAINDLGPV ETNAHLLRYD SVHGRFPKEV    60
   61 EVAGDTIDVG YGPIKVHAVR NPAELPWKEE NVDIALECTG IFTSRDKAAL HLEAGAKRVI   120
  121 VSAPADGADL TVVYGVNNDK LTKDHLVISN ASCTTNCLAP VAQVLNDTIG IEKGFMTTIH   180
  181 SYTGDQPTLD TMHKDLYRAR AAALSMIPTS TGAAKAVGLV LPELKGKLDG VAIRVPTPNV   240
  241 SVVDLTFIAK RETTVEEVNN AISEAANGRL KGILGYTDEK LVSHDFNHDS HSSVFHTDQT   300
  301 KVMEGTMVRI LSWYDNEWGF SSRMRDTAVA LGKLI

Number of amino acids: 335

Molecular weight: 36195.2

Theoretical pI: 5.88

Amino acid composition:

Ala (A)  34	 10.1%
Arg (R)  17	  5.1%
Asn (N)  16	  4.8%
Asp (D)  23	  6.9%
Cys (C)   3	  0.9%
Gln (Q)   4	  1.2%
Glu (E)  18	  5.4%
Gly (G)  25	  7.5%
His (H)  11	  3.3%
Ile (I)  22	  6.6%
Leu (L)  27	  8.1%
Lys (K)  17	  5.1%
Met (M)   7	  2.1%
Phe (F)   8	  2.4%
Pro (P)  12	  3.6%
Ser (S)  18	  5.4%
Thr (T)  27	  8.1%
Trp (W)   3	  0.9%
Tyr (Y)   7	  2.1%
Val (V)  36	 10.7%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 34

Atomic composition:

Carbon      C	      1596
Hydrogen    H	      2570
Nitrogen    N	       448
Oxygen      O	       490
Sulfur      S	        10

Formula: C1596H2570N448O490S10
Total number of atoms: 5114

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

The first table lists values computed assuming ALL Cys 
residues appear as half cystines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    26495   26727   26515   26150   25320
Abs 0.1% (=1 g/l)   0.732   0.738   0.733   0.722   0.700



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    26350   26600   26395   26030   25200
Abs 0.1% (=1 g/l)   0.728   0.735   0.729   0.719   0.696


Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 21.40
This classifies the protein as stable.



Aliphatic index: 98.36

Grand average of hydropathicity (GRAVY): -0.026